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In vivo studies have shown that S1P expression is increased and associated with pulmonary resistance in cigarette smoke-induced COPD mice [93]

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Archived from the original on August 2, 2012

It took a couple of years of experimenting with the contents of the e-liquid and the best method for heating it as well as how to miniaturize the whole contraption to make it more like smoking (its easy to imagine early versions looking more like a malfunctioning breathing apparatus)