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GHK-Cu is the human copper-binding tripeptide (glycyl-L-histidyl-L-lysine + copper) a natural signal for skin remodeling and repair whose levels decline with age

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Stranded cDNA was then prepared using iScript TM cDNA Synthesis Kit (Bio-Rad Laboratories, Inc, USA, Cat#1 70-8891) and the Programmable Thermal Controller, PTC-100 (MJ Research, Inc) following the protocol provided with the kit