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Relative expression levels were calculated using the 2^(-Ct) method (Livak and Schmittgen, 2001)

This model was used by our team for nanoparticle studies [24], and its BBB properties were further enhanced by targeting signaling pathways with cARLA [40]

For patients considering their options: if oxidative stress is clinically evident (ask your physician about inflammatory markers like hsCRP, liver enzymes, or oxidised LDL), glutathione or NAC supplementation is reasonable adjunctive support

Although these disorders are very rare, they underscore the fundamental link between disrupted glutathione homeostasis and pyroglutamate accumulation [75]