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Molecular cloning All plasmids were constructed using restriction enzyme digestion and ligation following standard protocols from New England Biolabs (NEB)

Benefits : Reduces intestinal inflammation Promotes gut lining healing Supports healthy microbiome Reduces permeability (leaky gut) Applications : Inflammatory bowel disease (supportive) IBS with inflammation Leaky gut syndrome Post-antibiotic gut damage Protocol : 1-2mg daily oral or subcutaneous, 8-12 weeks Combines with : BPC-157 (excellent gut peptide) Injectable GHK-Cu dosing Most effective route for systemic benefits

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It is often explored for stress management and cognitive clarity