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Cells were grown in LB medium with ampicillin, induced with 0.5 mM IPTG at 25 C for 1012 h, and harvested after cooling to 4 C

Al-Qahtani FA, Arafah M, Sharma B, Siddiqi NJ (2017) Effects of alpha lipoic acid on acrylamide-induced hepatotoxicity in rats

Hence, there is a need for fine-tuned engineering strategies that not only improve pharmacokinetic properties, but also enhance specific effector functions with a minimum of amino acid substitutions to reduce the risk of immunogenicity