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After incubation in indicated medium for 24 h, the diameter of each cell disc before and after incubation was measured and ratio was calculated

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It drives the transition from proliferation to differentiation and increases the number and length of multinucleated myotubes in a dosedependent manner.The mechanism is mainly related to NNMT inhibition, restored NAD levels, and activation of the SIRT1PGC1 pathway