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Indeed translocation of mTORC1 from the cytoplasm to the lysosome, a rich compartment in amino acids, is critical for mTORC1 activation ( per se is also able to regulate mTORC1 activity ( In brief, activation of the GCN2-ATF4 amino acid stress pathway and inhibition of protein synthesis through inhibition of mTORC1 demonstrates the strong proteogenic role played by cysteine in tumor cells

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The NRF2 silencing was achieved by infecting lentiviral particles produced by employing co-transfection of pLKO.1 shRNA and packaging vectors in HEK 293FT cells