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The protocol is extremely flexible and easily adjusted if there are varying volumes to be purified
Users should expect to see very small colonies for the first 24 hours after plating transformants
High Quality Data: High efficiency adapter ligation produces complex libraries that yield improved sequencing depth uniformity and better coverage with fewer zero coverage regions
zebrafish organs and scales
CircLigase-ssDNA Ligase, 1,000 U Chemicals The protocol is extremely flexibleThermostable ligase that catalyzes the intramolecular ligation (i. e. circularization) of ssDNA templates Intramolecular ligation of single stranded DNA ends containing 5' phosphates and 3' hydroxyl groups ATP dependent, catalytic enzyme Circularizes ssDNA >30 bases long Compatible with high temperature ligation reactions due to thermostability of CircLigase enzyme Applications Production of ssDNA templates for rolling circle replication or rolling
US$ 184.50
US$ 138.50