Botanical Garden Blot (Green Algae, Yeast, Barley, Sweet Corn, Rye Grass, Oat, Wheat, Soy, Mung, Sunflower, Cotton, Beet, Tomato, Spinach) size:1 Blot coli RNase H

coli RNase H

PGDF-R (Platelet Derived Growth Factor Receptor)

30% deionized water

Five µL of each reaction were resolved by agarose gel electrophoresis and visualized by staining with ethidium bromide

Botanical Garden Blot (Green Algae, Yeast, Barley, Sweet Corn, Rye Grass, Oat, Wheat, Soy, Mung, Sunflower, Cotton, Beet, Tomato, Spinach) size:1 Blot coli RNase HThe blot is prepared using total protein extracted from a variety of 5 10 day old, whole plant tissues. The premade blots are prepared with the appropriate GenLysate and 50g protein is loaded onto a 4 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved they are transferred to a PVDF membrane, using Efficient Western Transfer Buffer. The blots are ready to be blocked and probed with the