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Additionally, the intense regime uses larger, longer and deeper puffs. This intense smoking regime is also characterized by a different ISO standard, as of 2018: Of course, no machine smoking regime can reflect the individual variation in nicotine uptake among smokers and heated tobacco product users

Chem Res Toxicol 30(10):17331766 Sabbioni G, Castao A, Esteban Lpez M, Gen T, Mol H, Riou M, Tagne-Fotso R (2022) Literature review and evaluation of biomarkers, matrices and analytical methods for chemicals selected in the research program Human Biomonitoring for the European Union (HBM4EU)

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Step 3: Watch the LED display glow red to indicate active charging