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After protease digestion and centrifugation (1200 rpm, 5 min), PBECs were cultured in bronchial epithelial cell medium (#3211, ScienCell, San Diego, California, USA), supplemented with 100 U/ml penicillin, 100 g/ml streptomycin and 1 bronchial epithelial cell growth supplement (#3262, ScienCell, San Diego, California, USA) using poly-L-lysine-coated flasks and employed for experiments at passage 24 without mycoplasma contamination (Additional file 2: Fig

According to The Institute of Environmental Sciences and Technology[9], an appliance can only be considered a HEPA filter if it traps 99.97% of particles 0.3 microns or larger

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Scarlet Fire is perfect for those who seek a powerful, daring fragrance that embodies warmth and allure, making it an ideal choice for evening wear or special occasions