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R., Phang, S.-M., Ravi, S., & Aswathanarayana, R

19 ) method to profile adult and aged central nervous system neuronal populations from as few as 200 neurons per mouse 20

After completion, as monitored by UPLC and ESI-LCMS, the reaction mixture was diluted with buffer A, filtered and purified directly by preparative RP-HPLC, eluting with a linear gradient (see the Peptide cleavage and purification section)