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To make it big in hip-hop, you don't need to cruise around selling mixtapes from your car boot, invent an elaborate story about a torturous jail stint (which is later exposed as a sham), put out cigarettes on your torso to make it look like you have bullet wounds or offer to give Dr Dre regular foot massages with baby oil (although the latter may help)

Operate the lever with a smooth, steady pull to help the machine pack the tobacco evenly and avoid any blockages

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Methods and materials Cell culture The human bronchial epithelial cell line BEAS-2B cells were obtained from the Shao laboratory, which is affiliated with the Institute of Medicine at Zhejiang University in Hangzhou, China