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After cooling, the tube was centrifuged for 10 min at 4000 rpm and the supernatant's absorbance was read on a spectrophotometer at 535 nm

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While the clinical diagnosis of PD is based on the presence of defining motor features of bradykinesia, rest tremor and rigidity, additional motor symptoms like freezing of gait, postural instability, falls, dysarthria and dysphagia are key drivers of disability in advanced disease [3]