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Despite these policies, mid-sized cities continue to encounter challenges with implementation (Graham et al., 2019), often a result of soft market demand (Brewer and Grant, 2015), ample greenfield development opportunities (De Sousa, 2017), and inflexible planning regulations which constrain higher-density development (Nicol and Biggar, 2024)

Pyroglutamic acid (5-oxoproline), a glutamate cyclized derivative, also presented a higher abundance in OVX rat serum/urine samples that was significantly reversed by HPL therapy (Figure 3D, metabolite determination results are shown in Supplementary Tables S10)