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The most common causes include B12 supplementation (particularly injections), liver disease releasing stored B12, haematological disorders increasing B12-binding proteins, and chronic kidney disease reducing B12 excretion

At 1 mg/day, even the 50mg vial exceeds the 28-day window reconstitute half at a time, or step your daily dose up to burn the vial faster

For recreational CrossFit athletes, this is not a concern.

Russ J Bioorg Chem 44:4151 Ciuffreda P, Xynomilakis O, Casati S, Ottria R (2024) Fluorescence-Based Enzyme Activity Assay: Ascertaining the Activity and Inhibition of Endocannabinoid Hydrolytic Enzymes