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20 ml of concentrated HNO 3 (65%) and 3 ml of 30% H 2 O 2 were added, and the tobacco was digested in a pressurized microwave apparatus (Milestone UltraClave IV, Germany) under a pressure of 50 bars and at a temperature of 180 C for 15 min

Interestingly, nicotinamide doses used in these studies were also several-fold higher than nicotines median lethal dose (LD50), suggesting that nicotinamide lacks stimulatory and toxic effects through nAChRs 6-9

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