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For the total proteins, 500 l each of SuperSignal West Pico Luminol Enhancer Solution (Thermo Scientific), SuperSignal West Pico Stable Peroxide Solution (Thermo Scientific, SuperSignal West Femto Luminol/Enhancer Solution (Thermo Scientific) and SuperSignal West Femto Stable Peroxide Buffer (Thermo Scientific) were mixed and pipetted onto the nitrocellulose membrane