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The prepared solution was emulsified by high agitation (25,000 rpm for 4 min at 70 C) and then homogenized at the same temperature through employing nine homogenization cycles at 750 bars by a high-pressure homogenizer

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The type of solution used for dilution plays a critical role in: Maintaining peptide structure and stability Ensuring solubility in research applications Protecting peptides from degradation caused by oxidation, pH imbalance, or contamination Selecting the right solution for dilution depends on the properties of the peptide and the intended research use

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