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Even though Coomassie Blue staining showed heterogeneity in protein size, incubation with peptide-N4-( N -acetyl-beta-glucosaminyl) asparagine amidase (PNGaseF) , a peptide-endoglycanase that removes high mannose, complex and hybrid N -glycans from proteins, homogenized all isoforms to a sharper band of 2526 kDa, compatible with the predicted molecular weights (MW) of deglycosylated RBD (26.5 kDa, Fig

Orphan Drugs 7 (4), 181197