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Bright Yellow 2, BY-2) were cultivated continuously in a 5-L fermenter (Type 100e, Applicon Biotechnology, AC Schiedam, Netherlands) with a 20% packed cell volume at 26C in the dark in Murashige-Skoog liquid medium (Murashige and Skoog Basal Salt Mixture, Duchefa Biochemie, Haarlem, Netherlands) supplemented with 3% (w/v) sucrose, 1 mg/L thiamine-HCl, 0.2 mg/L 2,4 dichlorophenoxyacetic acid and 100 mg/L myo-inositol