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Published clinical evidence includes a Phase 1b multiple-ascending-dose trial reported by Enebo and colleagues (Lancet, 2021) and a Phase 2 trial in type 2 diabetes reported by Frias and colleagues (Lancet, 2023), both studying cagrilintide together with semaglutide

DNMT activity assay In accordance with the manufacturers instructions, we extracted nuclear proteins with an Active Motif Nuclear Extraction Kit (Active Motif, Carlsbad, USA) and evaluated DNMT activity with an EpiQui DNA Methyltransferase Activity/Inhibition Assay Kit (Epigentek, Farmingdale, USA) as reported previously 64