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The tissue inserts were transferred into 1 mL of fresh culture medium, which bathes the basolateral side of the tissue, and exposed to the apical surface to DA vapor for 1 h at 37 C (+5% CO 2 ) using vapor cups (MatTek)

Oral nicotine products, particularly the nicotine pouch brand of ZYN, has received significant media attention and congressional attention

The absolute decreases in Mori current and daily smoking prevalence in the two years from 2019/20 to 2021/22 were similar in magnitude to those for the 8-year period from 2011/12 to 2019/20

As a check, we did calculate the economic cost of tobacco use based on data of the general category tobacco and found that the cost differs only slightly from the estimates provided here