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Preclinical studies in rodent models have examined its interactions with nitric oxide signaling, angiogenic factors including VEGF, fibroblast migration, and collagen synthesis in various tissue types

At the end of the study, final blood samples were taken, and the animals were euthanized by cardiac injection

Proposed pathways include modulation of multiple neurotransmitter systems (influencing serotonin, dopamine, norepinephrine, GABA, glutamate levels), possible involvement of NMDA receptors, alpha-1 adrenergic signaling (e.g., potentiating pineal acetyltransferase activity), inhibition of somatostatin release via dopaminergic routes, reduced ACTH/cortisol for stress buffering, and stimulated growth hormone or somatoliberin secretion