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Bacteria collected from 100 ml culture were lysed in 1 ml lysis buffer consisting of 858 l PBS, 40 l 25 complete protease inhibitor cocktail (Hoffmann-La Roche, Basel, Switzerland) 1 l 1 M DTT, 50 l 10 mg/ml lysozyme, 1 l 1 10 4 U/ml DNA ase I, and 50 l 20% Triton X on ice for 30~60 min, and then subjected to sonication

(2024) 20:e2308753

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Unreviewed observational studies also reported lower COVID-19 mortality in countries with universal BCG vaccination, such as South Korea and Japan, compared with countries without such practice, including Italy and the United States (205)