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Observational studies have tracked the effects of caloric restriction on lean, healthy individuals, and demonstrated that moderate restriction (2230% decreases in caloric intake from normal levels) improves heart function and reduces markers of inflammation (C-reactive protein, tumor necrosis factor [TNF]), risk factors for cardiovascular disease (LDL cholesterol, triglycerides, blood pressure), and diabetes risk factors (fasting blood glucose, insulin levels)

Generally, yes, but it is always smart to check with a doctor if you take specific prescribed medicines

For topical applications, you might also want sterility testing, especially if the peptide will be applied to open or fissured skin

B12 injections are generally considered safe if youre deficient, and side effects are super rare