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Hummers and Offerman method In the past, the Hummers and Offerman methods have been the most usual method to create graphite oxide

A reduced GSH status can further compromise the defense against oxidative stress and increase the oxidative modification of proteins or enzymes, causing major changes in the secondary structure that result in the impaired metabolic stability and function of modified proteins or enzymes [78,97]

This format suits active people who are often on the move, or those who prefer an alternative to capsules and powders while keeping a clear nutritional organisation

Quantitative real-time polymerase chain reaction (PCR) analysis Total RNA was extracted using a TRIzol kit (Invitrogen, Carlsbad, CA, USA) and cDNA synthesis was performed using a cDNA synthesis kit (Fermentas Life Sciences) according to the manufacturer instructions