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Caspase 3/7 assay HCT116 cells were treated with indicated concentrations of C1-27 for 24 h

The Hcy induced redox imbalance can compromise aldosterone synthase (low aldosterone) and 11beta hydroxylase (low cortisol), both mitochondrial based (Hggstrm & Richfield, 2014)

Between 2002 and 2014, the proportion of liver transplant registrants 60 years old rose from 19% to 41%, largely driven by hepatitis C virus, MASLD, and hepatocellular carcinoma

The samples were processed using histological techniques and stained using routine Hematoxylin and Eosin stain for histopathological examinations, adhering strictly to standard protocol