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Measurement of the renal glutathione (GSH)/oxidized glutathione (GSSG) ratio and malondialdehyde (MDA) and superoxide dismutase (SOD) levels GSH and GSSG levels were measured by kits purchased from Beyotime (S0053, Shanghai, China), and MDA (thiobarbituric acid method) and SOD (hydroxylamine method) levels were measured by kits purchased from Jiancheng Bioengineering Institute (A003-1 and A001-1, Nanjing, China) in fresh mouse renal tissues according to the manufacturers instructions, as we described previously 29

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