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An amount of 2 mL of fecal slurry was immediately taken in duplicate: one replicate was immediately frozen at 80 C to have an unstimulated fecal slurry sample for the NGS analysis, whereas the other replicate was acidified with 0.1N HCI (Sigma-Aldrich, 50%/50% v/v), centrifuged at 4 C at 900 x g for 8 min, and filter-sterilized in a 0.22-m cellulose acetate filter

However, more human studies are needed to confirm whether supplementing with B5 might improve adrenal function

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