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Finally, the samples were prepared for Western blotting analysis by lysing the brain homogenates on ice for 300 minutes in 100 mL of a lysis buffer composed of 120 mM NaCL, 40 mM Tris (pH 8), and 0.1% NP40, followed by centrifugation

That's a significant limitation

Would you say to someone who eats meat and can absorb B12 (which a PA patient cant do because they have no intrinsic factor): Oh you cant eat meat to get your B12 until Monday even though you are B12 deficient on Friday? This is unreasonable

There, it can strengthen or rebuild the gut barrier, fight inflammation, and help ulcers and other damaged tissue heal