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The slices were kept in EB2 medium (DMEM/F12 containing 2% FBS, 1x MEM nonessential amino acids, 1x penicillin/streptomycin, 0.1 mM -mercaptoethanol and 2 mM L-glutamine), which was replaced every other day, on a rocker plate (60 rpm, 15 C tilt angle) placed in an incubator set at 37 C, 5% CO 2 , 20% O 2 and 80% humidity

Additionally, bystander activation can result from vaccine-induced immune stimulation, leading to the activation of autoreactive T and B cells

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Use the formula: (Desired dose Total peptide in vial) Total BAC water units 100