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Afterwards, membranes were stained with primary antibodies against pSTAT3 (1:1,000), STAT3 (1:1,000), GAPDH (1:10,000), Prx1 (1:1,000), Trx1 (1:1,000), and respective secondary antibodies

They measured the apparent levels of messenger RNA (mRNA) associated with urea cycle enzymes in the liver, evaluated the overall nitrogen balance, and hypothesized the nitrogen contents of various organs

Comprehensive evaluation of mitochondrial redox profile, calcium dynamics, membrane integrity and apoptosis markers in a preclinical model of severe penetrating traumatic brain injury