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10.1007/s12010-022-04287-8 74 ZanoniD

And summarize and organize the modeling conditions of cells and animals damaged by blue light, providing reference for subsequent blue light damage modeling experiments

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Briefly, cells were grown in YPD until logarithmic phase, harvested by centrifugation at 1000 g for 3 min at room temperature, and resuspended in 200 l of One-step-transformation buffer containing 40% polyethylene glycol 3350 (Sigma Aldrich, St