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Table 2 gives a comprehensive overview of the existing and experimental approaches for NRT

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It's important to note that disposable vapes come from Asia, where nic salt e-liquids are used, but the e-liquids are not held to the high-standard of UK made liquids

While initial efforts attempted to cleave off the 6His-MBP tag using overnight dialysis in buffer containing TEV protease, it was found that isolated ATCase was unstable in solution