Analyses of the enzymatic activities of AST and ALT were performed according to the manufacturers instructions (Sigma-Aldrich, Milan, Italy)
When used together, these peptides address aging from multiple anglesboth cosmetic and cellular
When total protein extracts were analyzed by SDS-PAGE, a protein overexpression was evident upon IPTG induction, showing a strong band of 18.9 kDa that correspond to the expected molecular weight for GpoA wt -His x6 (Fig
The X-ray-derived data of small molecular fragments in complex with TGR used in this study to design the compounds here described are available in the PDB under accession codes 6FTC, 6FMU, 6FMZ, 6FP4, 2X99, 2X8G, 2X8H, 6ZST, 6ZP3, 6ZLP, 6ZLB, 7B02, 7NPX, 2V6O, 6RTJ, 6RTO, 6RTM, and 3H4K