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The preservation of the active site architecture, notably the cysteine residue at position 36 (Cys 36 ), coupled with the marked reduction in enzymatic activity exhibited by the GpoA Cys36Ala variant relative to the wild-type enzyme, provides compelling biochemical support for the essential role of this residue in mediating GpoAs peroxidase function
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A previous study found that serum MDA concentration and serum C2C concentration (a marker of collagen type II degradation) were significantly increased in ACLT-induced OA dogs [96]
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