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Proteins were separated on Bis-Tris SDSPAGE, transferred onto nitrocellulose membrane and probed with anti-p-eIF2 (Cell Signaling Technology, 3597), anti-eIF2 (Cell Signaling Technology, 2103) anti-p-ACC (Cell Signaling Technology, 3661), anti-ACC (Cell Signaling Technology, 3662), anti--tubulin (Proteintech, 10068-1-AP) and anti--ActinPeroxidase antibody (Sigma-Aldrich, A3854)

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If you need to return to high-level athletics or heavy manual labor, discuss which option best supports your long-term performance goals