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Peptide-only protocols suit users with mild wrinkles responding well to gentle intervention, needle-phobic individuals avoiding injections, budget-conscious users prioritizing cost savings, those valuing natural appearance over maximum smoothness, preventive users in 30s-early 40s delaying intensive treatment, and anyone philosophically opposed to neurotoxin injection or muscle paralysis accepting modest results for excellent safety and convenience following gradual anti-aging approaches
Cells were incubated with BDNF or GSB-106 (in one or various concentrations, depending on the type of experiment) for different time intervals after starvation
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The barrier integrity of the model was verified by permeability measurements for the transcellular biomarker EBA and small hydrophilic paracellular reference molecule fluorescein
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