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Aug 2013;30(8):587-92

56:52 The Different Causes of Constipation 1:02:23 The Myth of One Bowel Movement a Day 1:05:42 Final Takeaways on Gut and Brain Health ===== Trisha Pasricha, MD, MPH is an instructor of medicine at Harvard Medical School and the Ask A Doctor columnist for The Washington Post where she translates complex medical topics into must-read insightswith a touch of humorfor millions each week

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After expression of roGFP2 for 16 h at 20 C, as aforementioned, cells were harvested, washed in HEPES buffer (40 mM, pH 7.4) and adjusted to an OD 600 of 1.0 in HEPES buffer containing either 1 mM Aldrithriol-2 (Sigma-Aldrich, Darmstadt, Germany, CAS-2127-03-9, AT-2) (oxidation control), 10 mM Dithiothreitol (Sigma-Aldrich, Darmstadt, Germany, CAS-3483-12-3, DTT) (reduction control), or buffer