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Weight loss was primarily from fat mass rather than lean mass, and the treatment was generally well-tolerated with no significant adverse effects on glucose metabolism, insulin levels, or IGF-1 levels

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Portions upstream (443 bp) and downstream (338 bp) of the GDH2 gene were amplified and fused with the 5269 bp SAT1 fragment to form a disruption cassette (~6 kb) for GDH2 gene deletion (Supplementary Fig