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Results: MTX altered the concentration of 31 out of 70 metabolites analyzed, suggesting inhibition of the glycine cleavage system, the pentose phosphate pathway, purine and pyrimidine synthesis, phospholipid metabolism, and bile acid uptake

Testing may include SAM, SAH, methionine, homocysteine, whole-blood histamine, copper, zinc, ceruloplasmin, urinary pyrroles, organic acids, lactate, pyruvate, glutathione-related markers, 8-OHdG and lipid peroxides when clinically appropriate

Y.GoerkeJ.KoushafarH.YagerD.KenagaL.et al (1995)

Suspension nasal sprays require all of the same device performance tests as solution sprays, plus drug particle size distribution (DPSD) by cascade impactor because it is the drug particle size (not the droplet size) that determines absorption rate in the nasal mucosa for suspensions