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Periodontal regeneration with peptide-enhanced anorganic bone matrix in particulate and putty form in dogs

Dissected cochlear epithelia are transferred to permeable membrane inserts (Millicell organotypic culture plate inserts, Millipore) which are placed into 1 mL of culture media (Dulbecco's modified Eagle's medium (high glucose, Glutamax, 25 mM HEPES) with 10% fetal bovine serum, 1% N2 supplement and 10 units/ml penicillin) in a 6-well plate

Prior to hydrolysis, an internal standard of Norvaline was added to each sample to achieve a final concentration of 250 mol/L

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