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Such a situation may have rendered this group of population even more susceptible to SARS-CoV2 at the time of its spreading

Then, 450 l of supernatant was collected and completely dried under vacuum using a SpeedVac vacuum concentrator (Savant SPD131DDA, Thermo Fisher Scientific, Waltham, MA, USA) at 45 C for 90 min at 1.5 atm pressure

HGF human growth factor HGF treatment-induced spines labeled with mRFP- -actin were immunostained for the universal presynaptic marker Synapsin that is located in the presynaptic active zones (Ferreira and Rapoport 2002) and the excitatory presynaptic marker VGLUT1 that is found at glutamatergic presynaptic synapses (Balschun, Moechars et al.)