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Optimization of culture conditions and medium composition for enhanced glutathione production To further enhance glutathione production, the effects of culture temperature, pH, and medium composition were evaluated using the #14 mutant with improved glutathione content

He also flags that glutathione contains a thiol group that oxidizes in water over time, converting the active form to an inactive one, and recommends using reconstituted vials within 14 days rather than the 28-day window common with other peptides

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