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Additionally, concerns regarding the risk of pancreatitis, thyroid disorders, and depression with the use of GLP-1 RAs require careful monitoring and further investigation

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Prior to any procedures, animals are anesthetized using a combination of xylazine (10 mg/kg), ketamine (40 mg/kg) and acepromazine (0.75 mg/kg) for up to one hour via the intramuscular route

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