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2002, 283: R29-R45

NAC is also an antioxidant itself as a source of sulfhydryl groups in cells and scavenger of free radicals as it interacts with reactive oxygen species such as OH and H 2 O 2 60

In this study, a robust hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method was developed and optimized for the comprehensive analysis of glutathione metabolism in cell samples without chemical derivatization