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Cells were seeded in advanced DMEM/F-12 (Thermofisher) supplemented with R-Spondin (10% v/v) and Noggin (10% v/v) conditioned media, B-27 supplement (Thermofisher) (1X), nicotinamide (10 mM) and FBS (1%)

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For example, ceruloplasmin is a copper-containing ferroxidase that possesses ferroxidase and antioxidant activity and inhibits iron-and copper-dependent lipid peroxidation and scavenging peroxide and superoxide anions

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