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Materials and methods Cell culture and treatments Human 21NT breast cancer cells were cultured in 1 Modified Eagles medium alpha (alpha MEM) (Gibco), 2.8 M hydrocortisone, 1 g/ml insulin, 10% foetal calf serum (FCS), 1% gluta- max, 10 mM HEPES, 0.1 mM NEAA and 12.5 ng/ml Epithelial Growth Factor (EGF)

Speculations on activation of CD4 by HIV gp120 Given the importance of CD4 CSD reduction in the entry of HIV gp120 into the cell, it is worth speculating on the nature of this process in the light of the hypothesis put forward here

The accumulation of these products is caused by impaired renal function, as seen in CKD (Evenepoel et al., 2017

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