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The animals were bred in the animal house of the Department of Biochemistry, Michael Okpara University of Agriculture, Umudike under standard conditions (2527 C, 12 h natural light-dark cycle) and fed with commercially available rat pellets and water ad libitum

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This hyperinsulinemia not only worsens insulin resistance but also contributes to metabolic disturbances, including dyslipidemia and an increased cardiovascular risk

doi: 10.1094/MPMI-05-10-0117 33 KalaposB.JuhszC.BaloghE.KocsyG.TbisI.GullnerG